In histology, signal molecules are studied using various techniques to understand their roles in cellular functions and tissue organization. Common methods include:
- Immunohistochemistry (IHC): This technique uses antibodies to detect specific signal molecules within tissue sections. It allows the visualization of the spatial distribution of these molecules in different cell types. - In Situ Hybridization (ISH): This method detects specific nucleic acid sequences, providing information on the expression patterns of genes encoding signal molecules. - Western Blotting: This technique is used to detect and quantify specific proteins, including signal molecules, in tissue samples.