In histology, the detection of ROS is crucial for understanding their role in disease pathology. Techniques such as immunohistochemistry and fluorescence microscopy are commonly used to visualize ROS levels in tissue samples. Specific dyes like DHE (dihydroethidium) and DCF-DA (2',7'-dichlorofluorescin diacetate) are employed to detect superoxide and hydrogen peroxide, respectively. These methods allow for the localization and quantification of ROS within different cellular compartments.
