Fixation: The tissue is fixed using chemicals like formaldehyde or glutaraldehyde to preserve cellular structures. Dehydration: Water is removed from the tissue using a series of ethanol baths, as water can interfere with resin infiltration. Infiltration: Dehydrated tissue is infiltrated with resin, which penetrates the tissue spaces and replaces the ethanol. Polymerization: The infiltrated tissue is placed in molds and polymerized, usually through heat or UV light, to harden the resin. Sectioning: The hardened resin block is trimmed and sectioned using a microtome or ultramicrotome for observation under a microscope.
