The detection of protein biomarkers in histology involves several techniques:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections, providing both qualitative and semi-quantitative data. Western Blotting: Although more commonly used for protein analysis in tissue homogenates, it can provide complementary data to IHC. Mass Spectrometry: Advanced techniques like MALDI-TOF and LC-MS/MS offer high sensitivity and specificity for protein detection and quantification. Fluorescence Microscopy: Uses fluorescent-tagged antibodies to visualize protein distribution within tissue samples.
