When selecting isotype controls, it is essential to match several characteristics of the primary antibody:
Host Species: The isotype control should be derived from the same species as the primary antibody. Isotype: The isotype (e.g., IgG1, IgG2a) should be identical to the primary antibody. Concentration: The concentration of the isotype control should match that of the primary antibody. Label: If the primary antibody is conjugated to a fluorophore or enzyme, the isotype control should carry the same label.
