In vitro studies in histology typically involve several key steps: 1. Tissue or Cell Isolation: Samples are obtained from organisms and carefully isolated to maintain their integrity. 2. Culture Conditions: Isolated cells or tissues are then placed in a culture medium that provides the necessary nutrients and conditions for survival and growth. 3. Experimental Manipulation: Various experimental conditions, such as drug treatments or genetic modifications, are applied to study their effects on the cells or tissues. 4. Analysis: Techniques like microscopy, immunohistochemistry, and molecular assays are used to analyze the cellular changes and responses.