The preparation of histological slides involves several critical steps. First, tissue samples are obtained through biopsies or surgical procedures. These samples are then fixed, usually in a solution like formalin, to preserve the tissue and prevent degradation. Following fixation, the tissue is embedded in paraffin wax to provide structural support. Thin sections, typically around 5 micrometers thick, are then cut using a microtome. These sections are mounted on glass slides and stained to highlight different cellular components.
