How are Histological Samples Prepared and Analyzed?
When a syphilis infection is suspected, tissue samples are often collected via biopsy. These samples are then fixed, usually in formalin, and embedded in paraffin. Thin sections are cut, mounted on slides, and stained using techniques such as Hematoxylin and Eosin (H&E). Special stains, like the Warthin-Starry stain, may be used to highlight spirochetes. Microscopic examination can then reveal the characteristic histopathological features of syphilis.