How are Cell Adhesion Molecules Studied in Histology?
Histologists use several techniques to study CAMs, including:
Immunohistochemistry (IHC): This technique involves using antibodies to detect specific CAMs in tissue sections. It helps localize the expression of these molecules within tissues. In Situ Hybridization (ISH): ISH allows for the detection of specific nucleic acid sequences within tissues, providing information about the expression of CAM-related genes. Electron Microscopy: This method provides detailed images of cell-cell and cell-ECM interactions at the ultrastructural level. Western Blotting: Although not a histological technique per se, Western blotting can be used to quantify the expression levels of CAMs in tissue extracts.
