How are Biochemical Properties Analyzed in Histology?

There are several techniques used to analyze biochemical properties in histology:
1. Histochemical Staining: This involves the use of specific stains that react with biochemical components in tissues. For instance, Hematoxylin and Eosin (H&E) staining is used to differentiate between acidic and basic tissue components.
2. Immunohistochemistry (IHC): IHC uses antibodies to detect specific antigens in tissues, allowing for the identification of proteins, enzymes, and other molecules.
3. Enzyme Histochemistry: This technique involves the localization of enzyme activity within tissues. An example is the detection of lactate dehydrogenase, which is involved in glycolysis.
4. In Situ Hybridization (ISH): ISH is used to detect specific nucleic acid sequences within tissues, providing insights into gene expression.