How are Biochemical Assays Performed in Histology?
Performing biochemical assays in histology typically involves several steps:
Tissue Collection and Preparation The first step is to collect and prepare tissue samples. This may involve fixation, embedding, and sectioning to preserve the tissue architecture and molecular integrity.
Homogenization Tissue samples are then homogenized to break down the cells and release their biochemical contents. Various homogenization techniques, such as mechanical disruption and sonication, can be used depending on the tissue type and the desired assay.
Assay Execution The homogenized tissue samples are subjected to specific biochemical assays according to standardized protocols. This often involves the addition of reagents that react with the target molecules, producing measurable signals.
Data Analysis The final step involves analyzing the data obtained from the assays. This typically includes normalization, statistical analysis, and interpretation of the results in the context of the histological study.
