How are Acid Phosphatases Detected in Histological Samples?
The detection of acid phosphatases in histological samples is typically performed using enzyme histochemistry techniques. One common method involves the use of a substrate that releases a colored product upon hydrolysis by ACP. For example, naphthol AS-BI phosphate can be used as a substrate, yielding a colored azo dye when ACP activity is present. This allows for the visualization of ACP activity in tissue sections under a microscope.
