The construction of 3D skin models involves layering different types of skin cells on a scaffold. Common methods include:
Air-liquid interface culture: In this method, keratinocytes are cultured at the interface of air and liquid to promote stratification and differentiation. Decellularized skin matrices: These provide a natural scaffold that preserves the extracellular matrix, promoting cell attachment and growth. Bioprinting: Advanced techniques like 3D bioprinting allow precise placement of multiple cell types to closely mimic the native skin architecture.
