The creation of 3D cellular structures involves several key techniques:
1. Scaffold-Based Methods: These use biodegradable materials to provide a framework for cells to attach, grow, and form tissues. Common scaffold materials include collagen, gelatin, and synthetic polymers. 2. Hydrogel Systems: Hydrogels are hydrated networks of polymers that can encapsulate cells and provide a supportive environment for tissue formation. They are highly biocompatible and can be engineered to mimic the extracellular matrix. 3. Bioprinting: This technique uses 3D printing technology to deposit cells and biomaterials layer-by-layer, creating complex tissue structures. Bioprinting allows precise control over cell placement and tissue architecture.