While mCherry is a powerful tool, it does have some limitations:
Tissue Penetration: Like other fluorescent proteins, mCherry fluorescence can be attenuated in thick or dense tissues due to light scattering and absorption. pH Sensitivity: mCherry fluorescence can be affected by pH changes, which may impact its performance in certain experimental conditions. Photobleaching: Although relatively stable, mCherry is still subject to photobleaching, especially under high-intensity illumination.
