Are There Any Limitations to Fluorescence Illumination?
Despite its many advantages, fluorescence illumination has some limitations:
Photobleaching - fluorescent dyes can lose their ability to emit light after prolonged exposure to excitation light. Autofluorescence - some tissues may naturally emit fluorescence, complicating the interpretation of results. Cost - high-quality fluorescent dyes and specialized microscopes can be expensive. Complexity - requires precise optimization of staining protocols and imaging settings.
