While DAB is widely used, there are some limitations and considerations to keep in mind:
DAB is a potential carcinogen, so appropriate safety measures, such as wearing gloves and working in a well-ventilated area, are necessary. The brown precipitate can sometimes obscure other tissue details, making it challenging to interpret results in complex samples. DAB staining can fade over time, although this is generally minimal compared to other chromogens. It is important to optimize the staining protocol to avoid non-specific binding and ensure reproducibility.
