What is RNAlater?
RNAlater is a reagent used for the stabilization and storage of
RNA in biological samples. It is particularly useful in
molecular biology and
histology for preserving the
integrity of RNA within tissues and cells, preventing RNA degradation without the need for immediate processing or freezing. This makes it very valuable in clinical and research settings where sample collection may not be immediately followed by analysis.
How Does RNAlater Work?
RNAlater works by permeating tissues to inactivate
RNases, the enzymes responsible for RNA degradation. By preserving the tissue architecture and RNA content, RNAlater ensures that the
mRNA levels remain stable, allowing for accurate downstream applications such as
quantitative PCR and
RNA sequencing.
Why is RNAlater Important in Histology?
In histology, the preservation of tissue samples is crucial for accurate
analysis and diagnosis. RNAlater allows researchers and clinicians to maintain sample quality without the need for immediate processing or cryopreservation. This flexibility is essential for studies involving
longitudinal studies, remote sample collection, or when immediate resources for processing are unavailable.
What are the Advantages of Using RNAlater?
Stability: Samples can be stored at room temperature for extended periods, which simplifies logistics and reduces the need for
cold chain logistics.
RNA Quality: The reagent efficiently stabilizes RNA, maintaining its integrity for accurate analysis.
Simple Protocol: Using RNAlater is straightforward, requiring only the submersion of samples in the solution.
Compatibility: It is compatible with a range of downstream applications such as
RT-PCR,
microarray analysis, and sequencing.
How is RNAlater Used in Research Studies?
RNAlater is widely used in research studies involving gene expression profiling, tissue
biopsies, and
genetic research. Its ability to stabilize RNA post-collection allows researchers to collect samples in the field and process them later without compromising RNA integrity. This is particularly useful in ecological and environmental studies where immediate processing facilities are not available.
Can RNAlater Be Used for Long-Term Storage?
Yes, RNAlater is suitable for long-term storage. Samples can be stored at room temperature for up to a week, at 4°C for up to a month, and indefinitely at -20°C or -80°C. This versatility makes it a preferred choice for many laboratories aiming to preserve samples over extended periods. Conclusion
In summary, RNAlater is a vital tool in histology and molecular biology for the preservation of RNA integrity in biological samples. While it offers numerous advantages, researchers must consider its limitations and suitability for their specific applications. Its role in facilitating accurate and reliable
scientific research cannot be understated, making it a staple in both clinical and research settings.
