What Role do Stains Play?
Stains are crucial for differentiating between various tissue components. They enhance contrast and allow specific features to be visualized. For example,
hematoxylin stains nuclei blue, while
eosin stains cytoplasmic components pink. Together, they form the widely used Hematoxylin and Eosin (H&E) stain.
What are the Safety Precautions?
Handling chemical reagents requires strict
safety precautions. Many of these substances are hazardous. For example, formaldehyde is a known carcinogen, and proper ventilation and protective equipment are essential. Disposal of chemical waste must follow regulatory guidelines to minimize environmental impact.
Conclusion
Chemical reagents are indispensable in histology, facilitating the detailed study of tissues. From fixation to staining and mounting, each reagent plays a specific role in preserving and highlighting tissue structures. With ongoing advancements, histology continues to evolve, providing deeper insights into cellular and molecular biology.
