The indirect method offers several advantages: 1. Signal Amplification: The use of a secondary antibody can amplify the signal, making it easier to detect antigens that are present in low quantities. 2. Flexibility: Researchers can use a variety of secondary antibodies to detect different primary antibodies, providing a versatile approach to multiple antigen detection. 3. Cost-Effectiveness: Since one secondary antibody can be used with different primary antibodies, it reduces the cost associated with the production of multiple labeled primary antibodies.
