The use of vacuum in histology serves multiple purposes:
1. Dehydration: During the dehydration phase of tissue processing, a vacuum helps to efficiently remove water from tissue samples, which is essential for proper infiltration of embedding media. 2. Embedding: In the embedding process, a vacuum ensures that the embedding medium thoroughly infiltrates the tissue, preventing the formation of air bubbles that could distort the sample. 3. Sectioning: A vacuum is used in some microtomes to hold samples steady, ensuring precision during the sectioning process.
