Incubation is a critical step in histology for several reasons:
Enzyme Activity: Certain histological techniques require enzymatic digestion to reveal specific structures or components within the tissue. Proper incubation ensures that enzymes function optimally. Antigen Retrieval: Immunohistochemistry often involves antigen retrieval, which requires precise incubation to unmask antigens and allow antibodies to bind effectively. Staining: Many staining protocols, such as the Hematoxylin and Eosin stain, require incubation to ensure that stains penetrate tissues adequately and bind to specific cellular components. Temperature Control: Incubation conditions, including temperature, humidity, and time, are carefully controlled to maintain tissue integrity and prevent degradation.
