Histological samples are often stained with multiple dyes to highlight different structures and components within the tissue. For instance, Hematoxylin and Eosin (H&E) staining is a common method where hematoxylin stains nuclei blue, and eosin stains cytoplasm and extracellular matrix pink. Without color deconvolution, distinguishing between these stains can be challenging, especially when they overlap. By separating the stains computationally, color deconvolution allows for more precise quantification and analysis of specific tissue components.
