Chemicals are essential in histology for several purposes: - Fixation: Chemicals like formaldehyde or glutaraldehyde are used to preserve tissue structure. - Staining: Dyes and stains such as hematoxylin and eosin are used to differentiate cellular components. - Dehydration and Clearing: Alcohols and xylene are used to prepare tissues for embedding. - Embedding: Paraffin wax or resins are used to embed tissues for sectioning.