Alcian Blue staining can be performed at different pH levels to differentiate between various types of acidic mucosubstances:
pH 2.5: At this pH, Alcian Blue stains both sulfated and carboxylated acidic mucopolysaccharides. pH 1.0: At this lower pH, it predominantly stains sulfated mucopolysaccharides.
The choice of pH allows for more specific identification of the targeted molecules within the tissue.
