What Techniques are Used to Detect Neuron Specific Enolase?
Several techniques are employed to detect NSE in histological samples, including immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), and western blotting. Among these, IHC is the most commonly used method in histology labs. In IHC, antibodies specific to NSE are applied to tissue sections, and the presence of NSE is visualized using chromogenic or fluorescent detection methods.