What Techniques are Used to Detect IL-10 in Tissue Samples?
Several techniques are employed to detect IL-10 in histological samples, including:
Immunohistochemistry (IHC): This technique uses antibodies specific to IL-10 to visualize its presence in tissue sections. In situ hybridization (ISH): This method detects IL-10 mRNA within cells, indicating active transcription of the IL-10 gene. Flow cytometry: While not strictly a histological technique, flow cytometry can be used on dissociated tissue cells to quantify IL-10 production.
