Several advanced techniques can be employed to enhance the visibility of [unstained tissues](#): - Phase-Contrast Microscopy: Enhances contrast by amplifying variations in the refractive index of the specimen. - Differential Interference Contrast (DIC) Microscopy: Uses polarized light to produce high-contrast images of unstained samples. - Fluorescence Microscopy: Utilizes fluorescent dyes or proteins to highlight specific structures within the tissue.