What Techniques are Used in Histology for Diagnostic Purposes?
Several techniques are employed in histology to aid in diagnostic pathology:
Hematoxylin and Eosin (H&E) staining: The most commonly used staining technique, providing a general overview of tissue architecture. Immunohistochemistry (IHC): Utilizes antibodies to detect specific antigens in tissues, helping to identify the presence of particular proteins. Special Stains: Techniques like Periodic Acid-Schiff (PAS) and Masson's Trichrome highlight specific tissue components, aiding in the diagnosis of diseases such as glycogen storage disorders and fibrosis. Electron Microscopy: Provides ultra-high resolution images of cellular structures, useful for diagnosing conditions at the sub-cellular level.
