What Techniques are Used in Histology for Diagnosis?
Several techniques are employed in histology to aid in diagnosis:
Hematoxylin and Eosin (H&E) Staining: The most common staining technique, used to differentiate between different tissue components. Immunohistochemistry (IHC): Utilizes antibodies to detect specific antigens in tissues, aiding in the identification of particular cell types or disease markers. In Situ Hybridization (ISH): Detects specific DNA or RNA sequences within tissue samples, useful for identifying genetic disorders. Special Stains: Such as PAS, Masson's Trichrome, and Silver Stains, used to highlight specific tissue components that are not easily discernible with standard H&E staining.
