Staining methods are pivotal for differentiating between various tissue components. Traditional stains like Hematoxylin and Eosin (H&E) remain the gold standard for general tissue examination. However, specialized stains such as immunohistochemistry (IHC) and in situ hybridization (ISH) have expanded the capabilities of histological analysis. IHC can highlight specific proteins within cells, while ISH allows for the localization of specific nucleic acid sequences, providing insights into gene expression and regulation.
