Pimonidazole is a 2-nitroimidazole derivative that functions as a hypoxia marker. Due to its ability to form covalent bonds with macromolecules in hypoxic cells, it serves as an essential probe for studying tissue hypoxia. When administered to living organisms, pimonidazole is selectively reduced and forms adducts in cells under low oxygen conditions, which can be detected using specific antibodies.
