What is Fluorescence Resonance Energy Transfer (FRET)?
Fluorescence Resonance Energy Transfer (FRET) is a powerful and sensitive technique used in molecular biology and histology to study the interactions between proteins or other biomolecules. FRET occurs when energy is transferred from an excited donor fluorophore to a nearby acceptor fluorophore without the emission of a photon. This transfer depends on the proximity of the two molecules, typically within 1-10 nanometers, making FRET an excellent tool for investigating molecular interactions and conformational changes at a very fine scale.
