What Histological Techniques are Vulnerable to Phthalate Contamination?
Several histological techniques can be compromised by phthalate contamination, including:
1. Tissue Fixation: Phthalates can interfere with the fixation process, leading to poor preservation of tissue morphology and compromising the integrity of cellular structures. 2. Staining Procedures: Contaminants can alter staining properties, leading to misleading results. For example, phthalates may affect the binding affinity of dyes and antibodies, skewing immunohistochemistry outcomes. 3. Microscopy: The presence of phthalates can create artifacts in microscopy images, making it challenging to distinguish between true cellular structures and contaminants.
