What Histological Techniques Are Used to Study the Effects of Oxygen Therapy?

Histological techniques are crucial in studying the effects of oxygen therapy. Some common methods include:

- Hematoxylin and Eosin (H&E) Staining: This standard staining technique allows for the examination of general tissue structure and the identification of cellular changes.
- Immunohistochemistry (IHC): IHC helps detect specific proteins associated with hypoxia, such as Hypoxia-Inducible Factor-1α (HIF-1α), providing insights into cellular responses to oxygen levels.
- Electron Microscopy: This technique offers detailed visualization of cellular organelles and can reveal ultrastructural changes due to oxygen therapy.