Tissue Type: Different tissues require distinct preparation methods. For instance, bone tissue needs decalcification, which adds to the setup time. Equipment: The availability and condition of equipment like microtomes, cryostats, and automated stainers can significantly impact setup time. li> Reagents: The type and quality of reagents, as well as their availability, can either streamline or delay the setup process. Sample Size: Larger sample volumes typically require more extensive preparation, thereby increasing setup time. Technician Skill: The expertise and experience of the histotechnologist play a crucial role in reducing setup time without compromising quality.
