Several factors can impact the consistency of stains:
Fixation: The type and duration of fixation can influence staining outcomes. Section Thickness: Inconsistent section thickness can lead to uneven staining. Staining Protocol: Variations in staining protocols, such as differences in reagent concentrations and incubation times, can affect consistency. Reagent Quality: The quality and age of staining reagents can lead to variability in staining.
