What Challenges are Associated with Histological Sectioning?
Histological sectioning can present several challenges:
Artifacts Artifacts are distortions or anomalies introduced during tissue processing. These can arise from improper fixation, embedding, or sectioning and can affect the interpretation of the tissueâs structure.
Section Thickness Achieving consistent section thickness is essential for accurate analysis. Variations can lead to differences in staining intensity and obscure important details.
Tissue Hardness Some tissues, such as bone, are inherently hard and difficult to section. Special techniques and equipment, such as decalcification for bone, may be required.
