Several factors can contribute to reduced detail in histological slides:
Sample Preparation: Poor fixation, improper sectioning, and inadequate dehydration can all lead to reduced detail. Staining Techniques: Incomplete or uneven staining can obscure important tissue features. Microscopy: Limitations in the resolution of the microscope or improper illumination can reduce detail. Slide Quality: Scratches, air bubbles, and debris on the slide can interfere with the observation.