There are several reasons why sterilization might be incomplete:
Improper Loading: Overloading or incorrect arrangement of items in an autoclave can hinder effective steam penetration. Insufficient Exposure Time: Not allowing enough time for the sterilizing agent to act can leave some microorganisms alive. Temperature Fluctuations: Inconsistent temperatures in the sterilization chamber can result in uneven sterilization. Equipment Malfunction: Faulty sterilization equipment can lead to incomplete cycles.
