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fluorescent labeling:
What are the Types of Fluorophores Used?
There are several types of fluorophores commonly used in fluorescent labeling, each with its own advantages and limitations. Some popular fluorophores include:
FITC (Fluorescein isothiocyanate)
: A green fluorophore with broad applicability.
TRITC (Tetramethylrhodamine)
: A red fluorophore often used in conjunction with FITC.
DAPI (4',6-diamidino-2-phenylindole)
: A blue fluorophore that binds specifically to DNA.
Alexa Fluor dyes
: A series of dyes available in a variety of colors, known for their high brightness and photostability.
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