What are the Techniques Used in Histological Examination?
Various techniques are employed in histology to examine tissues. Some of the most commonly used methods include:
Light Microscopy Light microscopy is the most basic and widely used technique. It involves staining tissue samples with dyes that highlight different cellular components, allowing for detailed examination under a microscope.
Electron Microscopy Electron microscopy provides much higher resolution images than light microscopy. It is used to study the ultrastructure of cells and tissues, revealing details at the molecular level.
Immunohistochemistry Immunohistochemistry involves using antibodies to detect specific proteins within tissues. This technique is particularly useful in identifying cancer markers and other disease-related proteins.
In Situ Hybridization In situ hybridization is used to detect specific nucleic acid sequences within tissues. This technique helps in identifying genetic abnormalities and the presence of viral RNA or DNA.
