The tissue sample undergoes several processing steps to prepare it for microscopic examination:
Fixation: Preserves tissue structure by using chemical agents like formalin. Embedding: Encases the tissue in a solid medium, usually paraffin, to facilitate thin slicing. Sectioning: Involves cutting the embedded tissue into very thin slices using a microtome. Staining: Enhances tissue contrast by applying dyes like hematoxylin and eosin (H&E). Mounting: Attaches the stained tissue slice onto a glass slide for microscopic examination.
