The process of staining chromaffin granules involves several steps:
1. Fixation: Tissue samples are fixed using chemicals like formaldehyde to preserve cellular structure. 2. Embedding: Fixed tissues are embedded in paraffin wax to create solid blocks that can be thinly sliced. 3. Sectioning: Thin sections of the tissue are cut using a microtome and mounted on glass slides. 4. Staining: Slides are treated with the chosen staining reagent (e.g., potassium dichromate) or antibodies in the case of IHC. 5. Visualization: Stained slides are observed under a microscope to identify and analyze chromaffin granules.
