Clarification is usually a part of the tissue processing workflow and involves several steps:
Dehydration: The tissue sample is first dehydrated using a series of alcohol baths to remove water. Clearing: The dehydrated tissue is then immersed in a clearing agent like xylene to replace the alcohol. Embedding: Finally, the tissue is embedded in a medium, typically paraffin, which supports the tissue structure and makes it easier to cut thin sections.
