The process of gel electrophoresis typically involves several steps:
Preparation of the gel: The gel is cast in a mold and allowed to solidify. Loading the samples: The samples are mixed with a loading buffer and then pipetted into the wells of the gel. Running the gel: An electric current is applied, causing the molecules to migrate through the gel. Staining and visualization: The gel is stained to make the separated molecules visible. Common stains include ethidium bromide for nucleic acids and Coomassie Brilliant Blue for proteins.
