What are the Steps for Using Chelex 100 Resin in DNA Extraction?
The general steps for using Chelex 100 Resin in DNA extraction from FFPE tissues include:
Deparaffinization of the tissue sample. Adding a suspension of Chelex 100 Resin to the sample. Incubating the mixture to allow the resin to bind metal ions. Heating the sample to denature proteins and release DNA. Centrifuging the mixture to separate the resin and other debris from the DNA-containing supernatant.
