What are the Steps for BrdU Labeling and Detection?
The process of BrdU labeling and detection typically involves the following steps:
BrdU Administration: BrdU can be administered via injection, drinking water, or directly added to cell culture media. Tissue or Cell Collection: After a suitable incorporation period, tissues or cells are collected and fixed. Denaturation: DNA is denatured to allow antibody access to BrdU. This can be done using acid, heat, or enzymatic treatment. Immunostaining: Specific antibodies against BrdU are applied, followed by secondary antibodies conjugated to a detectable marker (e.g., fluorescent dyes or enzymes). Visualization: The labeled cells are visualized using microscopy techniques such as fluorescence microscopy or confocal microscopy.
