There are several methods used for sectioning tissue, each with its own advantages and applications: 1. Paraffin Embedding: This is the most common method where tissues are fixed, dehydrated, cleared, and embedded in paraffin wax. Sections are typically cut using a microtome. 2. Cryosectioning: Tissues are rapidly frozen and sectioned using a cryostat. This method is faster and often used for immunohistochemistry. 3. Vibratome Sectioning: This technique is used for thicker sections needed for electrophysiological studies and involves cutting tissue using a vibrating blade.
