Despite its advantages, VVG stain also has some limitations:
1. Complex Procedure: The staining process is relatively complex and requires careful differentiation to avoid overstaining or understaining. 2. Time-Consuming: The entire procedure is time-consuming, which may not be ideal for routine diagnostic laboratories with high throughput. 3. Artifacts: The presence of artifacts due to improper fixation or staining can sometimes complicate the interpretation.
